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This Concept Map, created with IHMC CmapTools, has information related to: 02. DNA Analysis, run electrophoresis then transfer to a membrane, mutations cause fragments of different lengths implies mutations, deletions don't bind correctly, Abundant thus serve as good markers, electrophoresis requires 4 separate reactions, one for each nucleotide, small amounts of dideoxynucleotides ie ddATP, cleave with restriction enzymes note mutations cause fragments of different lengths, Production of chains with various lengths can be compared using electrophoresis, Production of chains with various lengths each ending with small amounts of dideoxynucleotides, mutations, deletions don't bind correctly includes neutral mutations, Restriction Fragment Length Polymorphisms (RFLPs) which serve as good markers, small amounts of dideoxynucleotides ie ddTTP, Production of chains with various lengths can be compared using flourescent dyes, Sanger method ingredients small amounts of dideoxynucleotides, Southern Blot spinoff northern blot, Format 2 theme Chip contains every time of mutation at every location, cleave with restriction enzymes then run electrophoresis, Format 1 theme probe cDNA is placed on glass, small amounts of dideoxynucleotides have no 2' or 3' O's, Southern Blot first Isolate entire genome from fetal cell, Sanger method ingredients DNA polymerase