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This Concept Map, created with IHMC CmapTools, has information related to: MEDIA (C-MAP), Methyl Red (MR) If Glucose is metabolized by Mixed Acid Fermentation, Acidic products are produced resulting in a low pH Positive=Red Color after Addition of methyl red indicator pHɜ.4 Enterobacteriaceae Description/Media, Urease: Breaks down urea, and ammmonia is releasedand pH increases. Change in color of pH is increased. Positive= change from Yellow to Pink UREA Enterobacteriaceae Description/Media, Description: Gram Stain: Nonspore forming, gram-negative rods Growth Characteristics: Faculative anaerobes Colonies on BAP: Large, Dull, Gray, Most are Non-Hemolytic Colonies on MAC: Lactose Fermenters Pink, Nonlactose Fermenters Colorless Enterobacteriaceae Description/Media, Carbohydrate fermentation Production of acidic end products causes a color change in the pH indicator. Phenol Indicator change is from Red to Yellow. ???? Enterobacteriaceae Description/Media, PDA Enterobacteriaceae Description/Media, Voges-Proskauer (VP) Acetoin is produced from an alternate pathway for glucose metabolism. Positive= Red Color when KOH & Alpha-Naphol are added. Enterobacteriaceae Description/Media, Triple Sugar Iron Agar (TSI) Sugar fermentation leads to acid production and change in color of pH indicator Yellow= Acid (A) Pink= Alkaline (K) Pink deep = glucose not fermentated Pink Slant= lactose/sucrose not fermented Yellow slant= Lactose and/or sucrose fermented Black precipitate= H2S production Bubbles=Gas TSI Enterobacteriaceae Description/Media, Citrate pH increases and the pH indicator changes color Positive=Change from Greene to Blue, or Growth Citrate Enterobacteriaceae Description/Media, H2S production Enzymes produce sulfur to the production of colorless gas. H2S reacts with iron salt to form ferrous sulfide Positive= Black precipitate H2S Enterobacteriaceae Description/Media, Decarboxylase rxn. Org. that possess the decarboxylate enzyme A.A. (Lysine, Arginine, Ornithine) pH increases & pH indicator changes color. Positive=change from Yellow to Purple Decarboxylase Rxn. Enterobacteriaceae Description/Media, Phenylalanine deaminase (PD) Deamniates Phenylalanine to Phenylpyruvic acid. Phenylpyruvic acid reacts with ferric chloride to produce a Green color Positive=Green Color after addition of Ferric Chloride PDA Enterobacteriaceae Description/Media, Motility If an org. is motile it grows away from the stab line in medium with an agar concentration 0.4% or < Positive = movement away from tthe stab line or a hazy appearance throught the medum Motility Enterobacteriaceae Description/Media, ONPG ONPG is changed to orthonitrophenol by betagalactosidase Positive=Yellow ???? Enterobacteriaceae Description/Media, Indole Enzyme Triptophanase deaminates tryptophan, with productproduction of Indole. Kovacs reagent forms a pink color=Positive Spot Indole= Blue Indole ????, Enterobacteriaceae Description/Media Oxidase: Tetramethyl-para-phenylenediamine diahydrochloride reacts with cytochrome C to produce blue or purple color =Positive, Nitrate Reduction If organism reduces nitrate to nitrate, a red color develops, when sulfanilic acid & N,N d-dimethyl-1-naphylamine are added Positive= Red, or no color after addition of zinc dust NITRATE Enterobacteriaceae Description/Media