WARNING:
JavaScript is turned OFF. None of the links on this concept map will
work until it is reactivated.
If you need help turning JavaScript On, click here.
This Concept Map, created with IHMC CmapTools, has information related to: Bioprocess, remove small amounts of cell debris and pumped through 0.22µ filter finished product is Clarified Lysate, Fermantion with broth and process is complete when most glucose is consumed and recorded by Batch Process Record a type is Intraceullar Fermination which the product of interest is kept inside the cell, Cell Lysis using Homogenizer (Cell Disruptors) and sent through 2 times lysed cells are pumped thorugh Centrifuges (LAPX 404), and Disc Stack Centrifuge to isolate the product., Solids and liquids: liquid is called Clarified Stream since almost all the solids have been removed which is waste and cells are in a paste form and put back through Centrifuges (LAPX 404), and Disc Stack Centrifuge to isolate the product., Clarified Lysate transfered by Lysate Tank- transfer vessel, Fermantion with broth and process is complete when most glucose is consumed and recorded by Batch Process Record next step is Cooldown and pump the product of interest into a broth tank, Chromatography ???? Hydrophobic-Interaction: interaction with water, Recovery with Bag Filters and Sieving to separate cells using Centrifuges (LAPX 404), and Disc Stack Centrifuge to isolate the product., 121 degrees celsius for 30 min using Sterilize in Place Cycle using SOPs to Remove Equipment and Materials, Purifcation a form of it is Chromatography, Cooldown and pump the product of interest into a broth tank harvest to start Recovery with Bag Filters and Sieving to separate cells, Product containing desired protein is known as Lysate starting Filtration using Microfiltration, Aseptic Vent Hood Station and build media and mix cell “feed stock�? Next is Placing feedstock in Bioreactor. Biotechnicans check for the temperature, pressure, pH level, oxygen, nutrient levels. Ingredients (media) added Yeast Extract, Tryptic Soy Broth, Ammonium Chloride Compound, Sodium Biphosphate, Monopotassium Phosphate, Antifoam Compound, Select Seed Stock from freezer transfer to Aseptic Vent Hood Station and build media and mix cell “feed stock�?, Centrifuges (LAPX 404), and Disc Stack Centrifuge to isolate the product. to separate Solids and liquids: liquid is called Clarified Stream since almost all the solids have been removed which is waste and cells are in a paste form, using permeable membranes to choose what is kept(filtration) which is Retentate: retained material to continue to loop to supply tank and continue filterating, Chromatography ???? Ion Exchange: based on charge difference, Centrifuges (LAPX 404), and Disc Stack Centrifuge to isolate the product. waste is removed Product containing desired protein is known as Lysate, Mixed Media in the reactor containg feed stock start Fermantion with broth and process is complete when most glucose is consumed and recorded by Batch Process Record, Sterilize the Equipment put Mixed Media in the reactor containg feed stock