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This Concept Map, created with IHMC CmapTools, has information related to: Chapter 3-5, Phenotype eg Immortalization, Subcutaneous is the location for injected tumor cells in Nude mice, Tumor viruses induce multiple changes in Phenotype, Phenotype eg High saturation density, Phenotype eg Anchorage independence, Phenotype eg Loss of contact inhibition, Phenotype eg Inability to halt proliferation in response to deprivation of growth factors, Tumorigenicity tested by host animals that are immunocompromised, Phenotype eg Increased transport of glucose, Tumorigenicity tested by host animals that are syngenetic, immunocompromised eg Nude mice, Phenotype eg Altered morphology, Phenotype eg Reduced requirement for mitogenic growth factors, Anchorage independence when in vivo indicates ability when in vitro to form tumors, Phenotype eg Tumorigenicity