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This Concept Map, created with IHMC CmapTools, has information related to: Class discussion 9-6-07, Transfection can be Stable transfection, Stable transfection has changes carried in all subsequent Generations, c-src likely has essential Function, Mutations are usually loss-of-function, Cell transformation involves Growth in absence of growth factors, Receptors detect Signals, Cell transformation involves Immortalization, Infected cells to make more Virus copies, Transfection can be Transient transfection, Stable transfection occurs by Recombination, Howard Temin was interested in how ssRNA, Isoforms can result from Alternative splicing, Cell transformation involves High saturation density, Reverse transcription led to techniques such as PCR, Proteins may exist as Receptors, Growth in absence of growth factors is related to other transformation properties as determined by Temperature sensitive mutants, Small viral genomes can be identified by use of Southern blot, Small viral genomes can be identified by use of PCR, Transfection often used 3T3 cells, Cell transformation involves Tumorigency