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This Concept Map, created with IHMC CmapTools, has information related to: Histological Methods, Chemically Fixed preserves Microstructure, Frozen in OCT, Embedding requires Dehydration, Chemically Fixed in Acid, Histotechnique prepares Tissue samples, Freeze-substitution for SEM, Plastic polymerizes to Harden, Cryostat preserves Lipids, Microtome to make Sections, Sectioning on Cryostat, Tissue samples can be Frozen, Frozen dehydrated Freeze-substitution, Chemically Fixed in Glutaraldehyde, Chemically Fixed in Alcohol, Ethanol miscible with Xylene, Slicing with a Microtome, Plastic resins including Methyl methacrylate, Paraffin wax used for Light Microscopy, Plastic typically 0.1-1 microns, Supportive Medium enables Slicing