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This Concept Map, created with IHMC CmapTools, has information related to: Microscopy1, Light Microscopy uses Photons, Chemistry e.g. Toluidine Blue, Illumination including Fluorescence, Photons to generate Contrast, Flourescence e.g. Widefield, Electron Microscopy include Transmission, Microscopy differ in Resolution, Flourescence e.g. Confocal, Chemistry e.g. Heavy metals, Affinity including Immunoglobulins (IHC), Illumination including Darkfield, Heavy metals for Transmission, Affinity including Nucleic acid complementarity (ISHH), Microscopy types Electron Microscopy, Microscopy types Light Microscopy, Histochemical staining based on Chemistry, Electrons to generate Contrast, Chemistry e.g. H&E, Histochemical staining based on Affinity, Tissue Sections of thickness 50-100 nm