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This Concept Map, created with IHMC CmapTools, has information related to: Viral diagnosis cmap IID 8_2015 presentation 1, Laboratory diagnosis of viral infections divided into 3 categories Serological Diagnosis, Virus isolation "gold standard" advantages can isolate range of viruses, pathogen detection depends on proper timing of specimen collection, Immunostaining disadvantages subjective, Electron Microscopy disadvantages requires expensive equipment and experienced observer, Hybridization probes advantages can detect most viruses, Immunostaining advantages inexpensive, Live animal inoculation ????, ???? nucleic acid Sequencing, Serological Diagnosis advantages very fast, Virus isolation "gold standard" advantages no false positives, Immunostaining advantages rapid, Electron Microscopy advantages able to observe viral particles, Virus isolation "gold standard" propagation systems Live animal inoculation, PCR advantages extreme sensitivity, Immunostaining disadvantages high complexity, Immunostaining advantages good sensitivity & specificity, Virus isolation "gold standard" disadvantages collection/transport conditions are critical, Virus isolation "gold standard" propagation systems Mammalian cell culture (primary cells, cell lines, organ cultures), Mammalian cell culture (primary cells, cell lines, organ cultures) Presence of growing virus is usually detected by induction of cytopathic effects or hemadsorption