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This Concept Map, created with IHMC CmapTools, has information related to: Fermentation, Pump to next process Pressure Sensor Tangential-Flow Filtration (Ultrafiltration Membrane), Bioreactor examples pH Meter, UV Optical Sensor(Checking for GFP) repeats to NaCl Solution to Release GFP from Beads, SecondTFF process goes to New Buffer, Lisate Transfer Vessel called Clarified Lysate in Transfer Vessel, Air Meter part of Clarified Lysate in Transfer Vessel, Centrifuge Suspended Cleaned Cells Homogenizer(Cell Disrupter), Centrifuge Clarified Stream Cell Paste(Solid), Homogenizer(Cell Disrupter) Raptured Cells Centrifuge, Pump to next process High Salt Eluate Concentrate GFP, 0.22 Micron Filter(remove the solids) Bacteria is 1 micron in diameter make up Clarified Lysate(Biological Soup), ++Beads in the Chromatograpy Column Non GFP solution is waste UV Optical Sensor (Checking for GFP), Clarified Lysate(Biological Soup) after that Lisate Transfer Vessel, Eluate(Product) then UV Optical Sensor(Checking for GFP), Tangential-Flow Filtration (Ultrafiltration Membrane) High Salt Eluate Ammonium Sulfate, Ammonium Sulfate make Permanent(Waste), Cell Paste(Solid) Suspeneded Cells in Cleaning solution Centrifuge, .22 Micron Filter finally Freeze Dry Package, Aspectic Hood Broth Bioreactor, Centrifuge Clarified Stream Discard the Liquid