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This Concept Map, created with IHMC CmapTools, has information related to: Biotechnology Processes, Conductivity Sensor Pressure Sensor 0.45 Micron Pre-Filter, Lysate lysate 0.22 Micron Filter, Bioreactor is instrumented with Spectrophotometer, Cell Paste (solid) Suspend Cells in Cleaning solution Cleaned Cell Paste, Clarified Lysate in Transfer Vessel inlet pump Conductivity Sensor, Bioreactor is instrumented with pH Meter, Eluate (Product) checks for GFP UV Optical Sensor, Anion-Exchange Chromatography --GFP is attracted to ++ Beads in the Chromatography, Centrifuge Broth Clarified Stream, Bioreactor broth Broth Tank, Bioreactor is instrumented with Glucose Analyzer, 0.45 Micron Pre-Filter Flow/Air Meter Filter Clarified Lysate, Collection Vessel forms High Salt Eluate, Ammonium Sulfate produces Retentate (Product), Column Chromatography exchanging buffers in the lysate Anion-Exchange Chromatography, Pressure Sensor high salt eluate ultrafiltrated Tangential-Flow Filtration, Suspended Cleaned Cells homogenizer (cell disrupter) Ruptured Cells, Second TFF process new buffer Final Filter, Final Filter .22 Micron Filter Move to Fill/Finish, NaCl Solution to Release GFP from Beads Eluate (Product)